Imaging Molecular Dynamics Symposium
The Imaging Molecular Dynamics symposium will highlight the application of Fluorescence Lifetime Microscopy and Fluorescence Fluctuation Spectroscopy Methods in biomedical research.
9:00-1:00 pm, Friday 23rd November 2018
Wright Theatre (C403), Medical Building (Bld 181), The University of Melbourne
Free and open to all. Registration has now closed.
Program: The plenary address will be given by Prof Enrico Gratton from the University of California, Irvine.
|9:00 - 9:30||Elizabeth Hinde||Quantitative imaging of the architectural organisation of the cell nucleus by fluorescence lifetime and correlation spectroscopy|
9:30 – 9:40
A biosensor based FLIM-FRET phasor approach to measure proteostasis capacity in cells
9:40 – 9:50
The dynamics and structure of 53BP1 foci in response to DNA double strand break
|9:50 - 10:00||Andreas Pannek||Unveiling the dynamics of the albumin-FcRn interaction by FCS and FLIM-FRET|
10:00 – 10:10
Using 2D pair correlation of molecular brightness to study oligomerization dynamics of transcription factors within live cells
|10:10 - 10:20||Sunyuan Zhang||Liquid droplet like behaviour of ataxin-1 nuclear bodies revealed by FFS|
10:20 – 10:30
Fluorescence anisotropy imaging microscopy: A new approach to study cell morphology
10:30 – 11:00
Morning tea sponsored by Olympus Australia
11:00 – 11:30
Prof. Trevor Smith
Time-Resolved Emission Microscopy of Optically Trapped and Photo-Aggregated Particles
11:30 – 12:00
Prof. Andrew Clayton
Correlating membrane protein activity with oligomeric state using lifetime, spatial autocorrelation and photobleaching
12:00 – 13:00
Prof. Enrico Gratton
Comprehensive correlation analysis (CCA) for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector.
Abstract: The Airyscan detector is used as a new concept in fluctuation correlation spectroscopy using superresolution. This detector which acquires data simultaneously on 32 detectors arranged in a hexagonal array is used as a nanocamera with frame rate in the range of one million fps. We exploit this detector for fluctuation methods based on time correlation at single points or at a number of points simultaneously, as well as methods based on spatial correlation in the area covered by the detector. We developed a Comprehensive Correlation Analysis of molecular fluctuations that allows the large number of users of the ZEISS LSM 880 to access current fluctuation methods in one single platform.