Imaging Molecular Dynamics Symposium

The Imaging Molecular Dynamics symposium will highlight the application of Fluorescence Lifetime Microscopy and Fluorescence Fluctuation Spectroscopy Methods in biomedical research. 


9:00-1:00 pm, Friday 23rd November 2018

Wright Theatre (C403), Medical Building (Bld 181), The University of Melbourne


Free and open to all. Registration has now closed.

Program: The plenary address will be given by Prof Enrico Gratton from the University of California, Irvine.




9:00 - 9:30Elizabeth HindeQuantitative imaging of the architectural organisation of the cell nucleus by fluorescence lifetime and correlation spectroscopy

9:30 – 9:40

Nagaraj Moily

A biosensor based FLIM-FRET phasor approach to measure proteostasis capacity in cells

9:40 – 9:50

Jieqiong Lou

The dynamics and structure of 53BP1 foci in response to DNA double strand break

9:50 - 10:00Andreas PannekUnveiling the dynamics of the albumin-FcRn interaction by FCS and FLIM-FRET

10:00 – 10:10

Ashleigh Solano

Using 2D pair correlation of molecular brightness to study oligomerization dynamics of transcription factors within live cells

10:10 - 10:20Sunyuan ZhangLiquid droplet like behaviour of ataxin-1 nuclear bodies revealed by FFS

10:20 – 10:30

Hamid Soleimaninejad

Fluorescence anisotropy imaging microscopy: A new approach to study cell morphology

10:30 – 11:00

Morning tea sponsored by Olympus Australia

11:00 – 11:30

Prof. Trevor Smith

Time-Resolved Emission Microscopy of Optically Trapped and Photo-Aggregated Particles

11:30 – 12:00

Prof. Andrew Clayton

Correlating membrane protein activity with oligomeric state using lifetime, spatial autocorrelation and photobleaching

12:00 – 13:00

Prof. Enrico Gratton

Comprehensive correlation analysis (CCA) for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector.

Abstract: The Airyscan detector is used as a new concept in fluctuation correlation spectroscopy using superresolution.  This detector which acquires data simultaneously on 32 detectors arranged in a hexagonal array is used as a nanocamera with frame rate in the range of one million fps.  We exploit this detector for fluctuation methods based on time correlation at single points or at a number of points simultaneously, as well as methods based on spatial correlation in the area covered by the detector.  We developed a Comprehensive Correlation Analysis of molecular fluctuations that allows the large number of users of the ZEISS LSM 880 to access current fluctuation methods in one single platform.