General Image Analysis Training and Consultation Request

Required fields are marked with an asterisk (*).

Personal Details

Full Name *

Email *

Position *

• Undergraduate or Honours Student
• MSc or PhD Student
• Research Staff (e.g. Causal staff, Research Assistant or Lab manager)
• Postdoctoral research staff (e.g. Research Fellow, lab head)

Supervisor's Full Name *

Department (or Institute if not UoM) *

Image data information

Please provide a short summary of your imaging project *

Which of the below best describes your sample (select all that are applicable)? *

• Tissue section
• Tissue wholemount
• Cleared tissue samples
• Cultured cells
• Organiod cultures
• Small model organisms (e.g. fish/fly)
• Other

Which instrument type did you use to acquire the data (please select all that apply)? *

• Widefield Microscope
• Confocal Microscope
• TIRF microscopy
• Lightsheet Microscope
• Multi-photon Microscope
• High-Content Imaging Microscope
• Laser Micro-dissection
• Super-Resolution Microscope
• Single Molecule Super-Resolution Microscope
• Unsure

What applications were performed (please select all that apply)? *

• z-stack (e.g. 3D acquisition)
• tile-scan
• multi-channel (1-4 channels)
• multi-channel (>4 channels)
• time series (<5 hours)
• time series (>5 hours)
• F-experiments (FRAP, FLIM, FRET)
• other

What level of image detail do you require (please select all that apply)? *

• Tissue level (e.g. 20x magnification or lower)
• Cellular level (e.g. 40x magnification)
• Sub-cellular level (e.g. 100x magnification, 250nm resolution)
• Super-resolution (e.g. 100x magnification, 120nm resolution)
• Single Molecule Super-Resolution (e.g. 100x magnification, 20nm resolution)
• Unsure

What is the average size of your image file? *

• 0 - 1 GB
• 1 - 10 GB
• 10 - 100 GB
• > 100 GB
• Unsure

If you can share your image file, we will have a look before we meet *

• Yes, please send me the upload link
• Yes, it is on the microscope computer (provide file path below).
• Yes, it is on my share drive (provide download link below)
• No, I don't have image to share yet

Provide the detail of file sharing e.g. location of your file on BOMP instrument or download link

Image analysis & Software

What do you require from this request? *

Please note that onsite access of the computers are not allowed due to the COVID19 safety procedure.

• Local Induction to Image analysis computer only (15 min)
• Local Induction and software training on Image analysis computer (2 hrs)
• Image analysis consultation (no need to access analysis computers yet) (1 hr)

Which software would you like to access (please select all that apply)? *

• Imaris
• Volocity
• Huygens Image Deconvolution
• FIJI / ImageJ
• Zeiss Zen
• Leica LAS X
• Nikon NIS element
• SimFCS
• Vistavision
• Other
• Unsure

What type of analysis would you like to perform (please select all that apply)? *

• Visualisation (e.g. making movie)
• File processing (e.g. airyscan processing/ deconvolution/stitching)
• Quantification (e.g. Counting/Area/Volume/Intensity)
• Colocalisation
• Filament tracing
• Tracking
• Other
• Unsure

Additional Comments

Questions for platform staff? *