Transmission Electron Microscopes

Krios G4

Bio21 institute (in partnership with the Walter and Eliza Hall Institute)

Flagship instrument for cryoEM single particle, routinely running at 300kV but with possibilities of changing to 200kV and 100kV on demand.

Equipped with an autoloader for high throughput sample obervation. Equipped with a Falcon 4 direct detector as well as a  Gatan bio quantum energy filter with a K3 direct detector and an upper CETA 4x4k CMOS camera. Also equipped with phase plate for increased contrast of small proteins.

The main applications of this microscope are:

  • Structural analysis of isolated particles (cryo EM single particle)
  • cryo tomography (3D) of cells, tissues or materials

Talos Arctica

Bio21 institute

Instrument for cryoEM single particle, routinely running at 200kV but with possibilities of changing between 100kV and 200kV on demand.

Equipped with an autoloader for high throughput sample obervation. Equipped with a Gatan quantum 965 energy filter with a K2 direct detector and an upper CETA 4x4k CMOS camera. Also equipped with phase plate for increased contrast of small proteins.

The main applications of this microscope are:

  • Structural analysis of isolated particles (cryo EM single particle)
  • cryo tomography (3D) of cells, tissues or materials

Glacios

Bio21 institute (Monash Parkville owned)

CryoEM single particle, routinely running at 200kV. Possibility of access two days per week.

Equipped with an autoloader for imaging of quick frozen samples. Equipped with a CETA 4x4k CMOS camer and a Falcon 4 direct detector.

The main application of this microscope are:

  • Structural analysis of isolated particles (cryo EM single particle)

Talos L120C

Bio21 institute

High throughput  cryoTEM instrument, routinely running at 120kV but with possibilities at 80kV on demand.

Equipped with CETA 4x4k CMOS camera and cryo box, fully automated controls

The main applications of this microscope are:

  • Negative staining
  • cryoEM screening
  • plastic sections

Tecnai F30

Bio21 institute

Flagship instrument, routinely running at 300kV but with possibilities of changing between 100kV and 300kV on demand.

Equipped with an anti-contaminator and cold stage which allows imaging of quick frozen samples as well as tomography at either room temperature or cryo conditions. Equipped with HAADF STEM detector and a Gatan quantum 965 energy filter amd an upper CETA 4x4k CMOS camera.

The main applications of this microscope are:

  • Structural analysis of isolated particles (cryo EM single particle)
  • Tomography (3D) of cells, tissues or materials at room temperature or in cryo conditions
  • Cryo EM of cells, tissues or materials
  • EFTEM
  • EELS
  • STEM tomography

Tecnai F20

Bio21 institute

High resolution instrument,routinely running at 200kV but with possibilities between 50kV and 200kV on demand.

Equipped with STEM and EDS detectors

The main applications of this microscope are:

  • Micro analysis for material and biological science
  • High resolution imaging