Resources

  • Societies
  • Web resources

    MyScope, microscopy tutorial (pre-requisite for one on one teaching at IHIC)

    Leica Science-Lab, tutorials, article about microscopy and applications

    EM University, large repository of knowledge on EM and cryoEM

    CryoEM101, videos and animation about the cryoEM workflow

  • Instrument manuals
  • Calculators

    Basic SPA box size calculator

    Assuming 2.7 mm Cs

    SPA refinement memory calculator

  • What to bring

    Tolls you will need:

    Minimum kit:

    - 2 sets of Dumont tweezers style #5, non-magnetic

    - appropriate grids (see below)

    - grid storage

    Optional tools:

    - ThermoFisher Vitrobot or  Leica EMGP tweezers

    - Diamond knife for ultrathing sections (35 degres for standard sections, 45 degres for hard materials) and cryo ultrathin sections (IHIC will provide glass knives only)

    Recommanded grid types by application:

    - negative staining and small nanoparticles: 400 mesh carbon coated grids (copper)

    - plastic sections; 50-100 mesh formvar carbon coated grids (copper)

    - plastic section tomography: 200 mesh formavar carbon coated grids (copper)

    - immunolabelling: 100-200 mesh formvar carbon coated grids (gold, nickel or copper)

    - routine cryoEM (liposome/exosome/cubosomes/phages/bacteria ...): 300 mesh lacey carbon (copper)

    - single particle analysis: 1.2/1.3 (or smaller holes) UltraFoil (or equivalent).

  • Calibrated pixel sizes

    If a map resolution is given the pixel size has been calibrated using ApoF, otherwise it is the standard TFS/Gatan calibration

    Krios Gatan K3  (likely to change slighty)

    Magnification (EFTEM mode)Pixel size (Å)Resolution of map used (Å)
    80,0001.0693.12
    105,0000.8332.33
    130,0000.6552.87
    160,0000.5092.56

    Krios Falcon 4 (likely to change slighty)

    MagnificationPixel size (Å)Resolution of map used (Å)
    96,0000.8191.88
       
       
      

    Arctica Gatan K2 (likely to change slighty)

    Magnification (EFTEM mode)Pixel size (Å)Resolution of map used (Å)
    79,0001.7283.5
    100,0001.342.7
    130,0001.0372.3
    165,0000.7912.5

    Tecnai F30 CETA

    MagnificationPixel size (Å)
    4,70023.6
    9,40012
    12,0009.4
    15,5007.14
    20,0005.56
    23,0004.71
    31,0003.51
    39,0002.79
    59,0001.82
    78,0001.4
    93,0001.17
    115,0000.94
    200,0000.47
    230,0000.39

    Talos L120C

    MagnificationPixel size (Å)
    2,60054.4
    3,40041.9
    5,30026.4
    6,70021.4
    8,50016.9
    11,00013.4
    13,50010.5
    17,5008.2
    22,0006.4
    28,0005
    36,0004
    45,0003.1
    57,0002.44
    73,0001.90
    92,0001.51
    120,0001.18
    150,0000.92
    190,0000.72
    240,0000.56
    310,0000.44
    390,0000.34