Resources
- Societies
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Web resources
MyScope, microscopy tutorial (pre-requisite for one on one teaching at IHIC)
Leica Science-Lab, tutorials, article about microscopy and applications
EM University, large repository of knowledge on EM and cryoEM
CryoEM101, videos and animation about the cryoEM workflow
- Instrument manuals
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Calculators
Basic SPA box size calculator
Assuming 2.7 mm Cs
SPA refinement memory calculator
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What to bring
Tolls you will need:
Minimum kit:
- 2 sets of Dumont tweezers style #5, non-magnetic
- appropriate grids (see below)
- grid storage
Optional tools:
- ThermoFisher Vitrobot or Leica EMGP tweezers
- Diamond knife for ultrathing sections (35 degres for standard sections, 45 degres for hard materials) and cryo ultrathin sections (IHIC will provide glass knives only)
Recommanded grid types by application:
- negative staining and small nanoparticles: 400 mesh carbon coated grids (copper)
- plastic sections; 50-100 mesh formvar carbon coated grids (copper)
- plastic section tomography: 200 mesh formavar carbon coated grids (copper)
- immunolabelling: 100-200 mesh formvar carbon coated grids (gold, nickel or copper)
- routine cryoEM (liposome/exosome/cubosomes/phages/bacteria ...): 300 mesh lacey carbon (copper)
- single particle analysis: 1.2/1.3 (or smaller holes) UltraFoil (or equivalent).