Resources
- Societies
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Web resources
MyScope, microscopy tutorial (pre-requisite for one on one teaching at IHIC)
Leica Science-Lab, tutorials, article about microscopy and applications
EM University, large repository of knowledge on EM and cryoEM
CryoEM101, videos and animation about the cryoEM workflow
- Instrument manuals
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Calculators
Basic SPA box size calculator
Assuming 2.7 mm Cs
SPA refinement memory calculator
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What to bring
Tolls you will need:
Minimum kit:
- 2 sets of Dumont tweezers style #5, non-magnetic
- appropriate grids (see below)
- grid storage
Optional tools:
- ThermoFisher Vitrobot or Leica EMGP tweezers
- Diamond knife for ultrathing sections (35 degres for standard sections, 45 degres for hard materials) and cryo ultrathin sections (IHIC will provide glass knives only)
Recommanded grid types by application:
- negative staining and small nanoparticles: 400 mesh carbon coated grids (copper)
- plastic sections; 50-100 mesh formvar carbon coated grids (copper)
- plastic section tomography: 200 mesh formavar carbon coated grids (copper)
- immunolabelling: 100-200 mesh formvar carbon coated grids (gold, nickel or copper)
- routine cryoEM (liposome/exosome/cubosomes/phages/bacteria ...): 300 mesh lacey carbon (copper)
- single particle analysis: 1.2/1.3 (or smaller holes) UltraFoil (or equivalent).
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Calibrated pixel sizes
If a map resolution is given the pixel size has been calibrated using ApoF or orientated gold , otherwise it is the standard TFS/Gatan calibration
Krios Gatan K3 (likely to change slighty)
Magnification (EFTEM mode) Pixel size (Å) Resolution of map used (Å) 81,000 1.069 3.12 105,000 0.833 2.33 130,000 0.655 2.87 160,000 0.509 2.56 Krios Falcon 4
Magnification Pixel size (Å) Resolution of map used (Å) 75,000 1.036 gold 96,000 0.808 gold 120,000 0.639 gold 155,000 0.508 gold Arctica Gatan K2 (likely to change slighty)
Magnification (EFTEM mode) Pixel size (Å) Resolution of map used (Å) 79,000 1.71 3.5 100,000 1.33 2.7 130,000 1.03 2.3 165,000 0.788 2.5 Tecnai F30 CETA
Magnification Pixel size (Å) 4,700 23.6 9,400 12 12,000 9.4 15,500 7.14 20,000 5.56 23,000 4.71 31,000 3.51 39,000 2.79 59,000 1.82 78,000 1.4 93,000 1.17 115,000 0.94 200,000 0.47 230,000 0.39 Talos L120C
Magnification Pixel size (Å) 2,600 54.4 3,400 41.9 5,300 26.4 6,700 21.4 8,500 16.9 11,000 13.4 13,500 10.5 17,500 8.2 22,000 6.4 28,000 5 36,000 4 45,000 3.1 57,000 2.44 73,000 1.90 92,000 1.51 120,000 1.18 150,000 0.92 190,000 0.72 240,000 0.56 310,000 0.44 390,000 0.34
