What to bring

Tolls you will need:

Minimum kit:

- 2 sets of Dumont tweezers style #5, non-magnetic

- appropriate grids (see below)

- grid storage

Optional tools:

- ThermoFisher Vitrobot or  Leica EMGP tweezers

- Diamond knife for ultrathing sections (35 degres for standard sections, 45 degres for hard materials) and cryo ultrathin sections (IHIC will provide glass knives only)

Recommanded grid types by application:

- negative staining and small nanoparticles: 400 mesh carbon coated grids (copper)

- plastic sections; 50-100 mesh formvar carbon coated grids (copper)

- plastic section tomography: 200 mesh formavar carbon coated grids (copper)

- immunolabelling: 100-200 mesh formvar carbon coated grids (gold, nickel or copper)

- routine cryoEM (liposome/exosome/cubosomes/phages/bacteria ...): 300 mesh lacey carbon (copper)

- single particle analysis: 1.2/1.3 (or smaller holes) UltraFoil (or equivalent).